Composite

Part:BBa_K3514007:Design

Designed by: Lukas Pranter, Oona Jung   Group: iGEM20_BOKU-Vienna   (2020-10-25)


T7 Bacteriophage Major Capsid Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7lacO promoter and B1001 terminator from registry; silent mutations for golden gate cloning-> see BBa_K3514006


Source

KEGG database, entry: 1261026

References

NCBI Reference Sequence: NP_041998.1

Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7. Ionel A., Velazquez-Muriel J.A., Luque D., Cuervo A., Caston J.R., Valpuesta J.M., Martin-Benito J., Carrascosa J.L. J. Biol. Chem. 286:234-242(2011)